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KMID : 0382619840040010321
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Hanyang Journal of Medicine
1984 Volume.4 No. 1 p.321 ~ p.330
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A Study of Crude Exotoxin of Pseudomonas aeruginosa
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朴光雄/Park, Kwang Woong
趙陽子/徐仁銖/Cho, Yang Ja/Suh, Inn Soo
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Abstract
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In recent years the exotoxin of Pseudomonas aeruginosa infection have been observed as an important invasive factors of the toxic substances. So I prepared and isolated the exotoxin from the cultural filtrates of Pseudomonas aeruginosa PA-103 strain, and observed the biologic activity for crude exotoxin.
Strain PA-103 was grown in a dialyzed medium of trypticase soy broth enriched with 1% glycerol and 1 M monosodium glutamate and then immediately after centrifugation,zinc acetate was added to thd supernatant fluid. The mixture was left for a few hour and theh centrifuged to collect the precipitate, which was dissolved in sodium citrate, and then obtained the crude PA-103 strain exotoxin.
When mice lethal toxicity of Pseudomonas aeruginosa PA-103 strains was inoculated into the peritoneal cavity, LD?? was 10??. The supernatant fluid (18 hours) of this strain was showed the 40% low lethal rate on undiluted concentration but LD?? of crude exotoxin was 44.54㎍.
Immunization of rabbit with crude exotoxin resulted in development of antitoxic serum. This antitoxic serum was showed distinctly gel diffusion pattern with Ouchterlony method but precipitate valules was low as 1:16.
Skin responses of rabbit with the crude exotoxin was showed the reddish induration and necrosis etc. 1 Minimum Red Dose (MRD) was below 0.125㎍.
Crude exotoxin of antitoxic neutralization in the skin responses of neutralization test in vitro neutralized the LD?? as 1:16, and in vivo skin neutralization responses was slightly below than this.
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